This one-day symposium is dedicated to the single-cell sequencing technology, which allows the simultaneous analysis of cell populations at the DNA and/or RNA level. It will offer an overview of its latest advancement and applications with a focus on the bioinformatics and biostatistics challenges raised by this type of data. The panel of speakers will discuss how to address these limitations. This symposium also aims to support the growing interest in such technology by stimulating interactions and collaborations.

• 9h30 : Welcome coffee

• 10h15-10h30: Opening

• 10h30-11h30: An integrative view of Single-cell Technology, Fabien Delahaye (Institut Pasteur de Lille, EGID, Lille)

• 11h30-12h20: Statistical challenges for analysis of RNA-Seq Single-Cell data, Laurent Modolo (CNRS, LBMC, Lyon)

• 12h20-13h30: Lunch

• 13h30-14h20: Single cell isoform profiling, 10xGenomics scRNA-seq and Nanopore long read sequencing, Kevin Le Brigand (CNRS, IPMC, UCAGenomiX, Nice)

• 14h20-15h10: Identification of rare sub-populations and reproducibility in scRNASeq, Akira Cortal (Clinical Bioinformatics lab, Institut Imagine, Paris)

• 15h10-16h: Single-cell RNA-seq analysis pipelines using the Eoulsan Workflow engine, Morgane Thomas-Chollier (IBENS, Paris) [PDF]

• 16h00: Coffee and cakes

Registration is free, but mandatory: form (deadline May 30). It covers coffee breaks and lunch.

The symposium will take place in “Pole Recherche” amphitheater D at the Faculty of Medicine of Lille University. The nearest metro station is “CHU - Centre Oscar Lambret” (yellow line, direction CHU Eurasanté): general access.

Fabien Delahaye, Martin Figeac, David Hot, Guillemette Marot, Céline Poux, Hélène Touzet